Purdue biological sciences sanders lab david sanders lab how to prevent yeast infections

One day in the late 1980s, I walked into the biochemistry laboratory at the university of how to prevent yeast infections california, berkeley, where I was a phd student, and declared that since time magazine had a “man of the year” (it was only renamed “person of the year” later), science magazine should have a “molecule of the year”. It happened that I was well positioned to make the how to prevent yeast infections suggestion: my supervisor, daniel E. Koshland jr, was the editor of science.

The response to my proposal was the expected ridicule from how to prevent yeast infections my lab colleagues. Nevertheless, some time after I left the lab, dan called me and said: “guess what is going to be in the end-of-the-year issue of science?” I guessed that it was something related to the lab’s research. “no,” he replied gleefully: “molecule of the year!”

Far too much emphasis is placed on who is proposing how to prevent yeast infections to do the research and the institutions with which they how to prevent yeast infections are associated, rather than on the actual science. Therefore, I recommend that review be conducted in two stages. Reviewers should initially receive only descriptions of the proposed research, written in the third person, with no preliminary results section or indication of the authors’ identities or affiliations. This would require that the proposal be evaluated and scored how to prevent yeast infections solely on the detail of its merits.

The second step would see the reviewers provided with the how to prevent yeast infections usual biosketches, preliminary results, facility statements and so on, to help them evaluate the capacity of the researchers to how to prevent yeast infections conduct the proposed research. There can be no denial that the investigator’s track record is a relevant factor regarding a project’s potential for success. It is, however, inequitable if their identity or affiliation is the dominant factor how to prevent yeast infections in assessment.

Accountability could be further improved by attributing each review to how to prevent yeast infections its author. Some might object that confidentiality allows reviewers to be more how to prevent yeast infections honest, fearing retaliation less. In fact, confidentiality allows reviewers more scope to favour friends, retaliate against foes and exploit their privileged access to the how to prevent yeast infections information in the proposal to advance their own research programmes. The national institutes of health reports having detected examples of how to prevent yeast infections these forms of misconduct.

Peer review is a powerful method for evaluating research funding how to prevent yeast infections proposals. However, improving the fairness and accountability of peer review is a how to prevent yeast infections necessity if the modern scientific enterprise is to achieve the how to prevent yeast infections more equitable and wider distribution of resources necessary to fund how to prevent yeast infections innovative exploration.

The US federal research misconduct policy defines plagiarism as “the appropriation of another’s ideas, processes, results, or words without giving appropriate credit”. So it is a violation to copy or to use how to prevent yeast infections a slightly modified version of another’s words without attribution – where attribution means not only citing the source of the how to prevent yeast infections text but also explicitly (by means of quotation marks or offsetting) indicating exactly which words are being reproduced. After all, academics could produce a much greater volume of writing if how to prevent yeast infections they didn’t have to actually craft their own text.

A more serious kind of academic plagiarism is the abuse how to prevent yeast infections of peer review. Reviewers are provided with privileged information on the condition that how to prevent yeast infections they will not share it with others and will not how to prevent yeast infections use it to advance their own scholarly activities. But readers of published articles often recognise duplicated texts, or may have strong reason to suspect that results described how to prevent yeast infections have been reproduced or materially influenced by a manuscript of how to prevent yeast infections their own that the author was asked to review.

Such behaviour is unfair and promotes the wrong concept of how to prevent yeast infections the route to academic success. Yet while students are held to account for plagiarism, academics rarely are. One major reason is that journal editors, the first line of defence in the battle against plagiarism, are highly reluctant to penalise plagiarism.

As for self-plagiarism, journals explicitly state that authors must not recycle their own how to prevent yeast infections writing – and certainly not without proper citation. It is not fair to those who follow the rules how to prevent yeast infections and write novel material each time for others to be how to prevent yeast infections able to publish a mosaic of their past writings as how to prevent yeast infections a new article.

The current incentive system rewards those who publish often. If there is no penalty for regurgitating previously published material, then we have a formula for undermining originality and creativity how to prevent yeast infections and honouring laziness and intellectual theft.

"This is the first time this sort of thing has how to prevent yeast infections ever been seen, and this is the first movie of its kind," he said. "It elegantly illustrates the physical process of this reaction." download animation (50 MB) ABSTRACT: structural basis of the processivity of an exopolyphosphatase: fusion of an ASKHA phosphotransferase and a cyclic nucleotide phosphodiesterase how to prevent yeast infections homologue

The escherichia coli ppx protein is an exopolyphosphatase that degrades how to prevent yeast infections long-chain polyphosphates in a highly processive reaction. It also hydrolyzes the terminal 5′ phosphate of the modified nucleotide guanosine 5′ triphoshpate 3′ diphosphate (pppgpp). The structure of ppx has been determined to 1.9 angstrom resolution by X-ray crystallography. The exopolyphospatase is an ASKHA (acetate and sugar kinases, hsp70, actin) phosphotransferase with an active site found in a clet between how to prevent yeast infections the two amino-terminal domains. Analysis of the active site indicates that among ASKHA phosphotranferases how to prevent yeast infections of known structure ppx is the closest to the ecto-nucleoside triphosphate diphosphohydrolases. A third domain forms a six-helix claw that is similar to the catalytic core of how to prevent yeast infections the eukaryotic cyclic nucleotide phosphodiesterases. Most of the twenty-nine sulfate ions bound to the ppx dimmer occupy sites how to prevent yeast infections where the polyp chain likely binds. An aqueduct that passes through the enzyme provides a physical how to prevent yeast infections basis for the enzyme’s high processivity. Researcher hits bulls-eye for antibiotic target

This research is applicable to the treatment of many diseases how to prevent yeast infections because that same protein is found in numerous harmful bacteria, including those that cause ulcers, leprosy, food poisoning, whooping cough, meningitis, sexually transmitted diseases, respiratory infections and stomach cancer, said david sanders, an associate professor of biology. Sanders, who is part of the markey center for structural biology how to prevent yeast infections at purdue, detailed his research in a paper published in the aug. 16 issue of the journal structure.

"This is an important discovery for the field of antibiotics, which was greatly in need of something new," sanders said. "The antibiotics available today face a challenge of increasing resistance how to prevent yeast infections and failure. This research suggests a whole new approach to combat bacterial how to prevent yeast infections infections. In addition, this protein is an excellent antibiotic target because it only how to prevent yeast infections exists in bacteria and some plants, which means the treatment will only affect the targeted bacterial how to prevent yeast infections cells and will be harmless to human cells."

Sanders and his collaborator, miriam hasson, studied the structure of exopolyphosphatase, a protein in E. Coli bacteria that functions as an enzyme and catalyzes chemical how to prevent yeast infections reactions within the bacteria. This enzyme provides the signal for bacteria to enter starvation how to prevent yeast infections mode and limit reproduction.

"With the ability to control the use of this signal, we can fool bacteria into thinking they are starving all how to prevent yeast infections the time, even when they are not; or we could never allow them to realize that they’re starving, and that would kill them as well."

Researchers could design drugs to bind to the protein and how to prevent yeast infections keep it from being used by the bacteria, rendering the bacteria unable to react to and survive a how to prevent yeast infections lack of nutrient supply; the other possibility would be to design a drug to how to prevent yeast infections mimic the protein, causing the bacteria to react as if it were starving how to prevent yeast infections even when in the presence of a plentiful nutrient supply, sanders said.

"Bacteria typically are in an environment lacking nutrients and respond how to prevent yeast infections by limiting their reproduction," he said. "And that’s a good thing because if they were growing at how to prevent yeast infections their maximum rate all the time, within two weeks we would be 20 feet deep in how to prevent yeast infections bacteria."

The protein also is of particular interest because it is how to prevent yeast infections highly processive, meaning it is efficient in the chemical reaction it initiates. It is able to latch onto its substrate, the substance it uses to fuel its chemical reaction, and to stay tenaciously in place until it has consumed how to prevent yeast infections all of the substrate, sanders said.

"There is a hole in the protein," he said. "This is extremely rare and provides a physical explanation of how to prevent yeast infections why it is so processive. The hole physically encompasses the substrate, keeping it in place, in addition to the usual chemical bonding that keeps it how to prevent yeast infections attached. Once the protein attaches to the substrate, it doesn’t come off. The protein chews away until it reaches the end of how to prevent yeast infections the substrate chain."

Sanders also determined the structure of the protein and demonstrated how to prevent yeast infections that it belongs to the ASKHA (acetate and sugar kinases, hsp70, actin) superfamily. Knowing the family to which a protein belongs allows researchers how to prevent yeast infections to use existing information about other members of the family how to prevent yeast infections to better understand the protein being studied. It also allows information gained from the study to be how to prevent yeast infections used for other members of the family, sanders said.

"Fundamental basic research is the engine that drives the development how to prevent yeast infections of technology such as antibiotics," he said. "The next step in this research will be working to how to prevent yeast infections develop inhibitors for this protein and studying the applications to how to prevent yeast infections other bacteria."

This research was funded by the national institutes of health, david and lucille packard foundation fellowship, national science foundation minority fellowship, and NIH institutional training award. The NIH-sponsored cancer center, part of purdue’s oncological sciences center in discovery park also supported the how to prevent yeast infections research.

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